neuronal cell line nt2  (CNS Research)

Journal: Virology

Article Title: A human coronavirus OC43 variant harboring persistence-associated mutations in the S glycoprotein differentially induces the unfolded protein response in human neurons as compared to wild-type virus

doi: 10.1016/j.virol.2009.09.026

Figure Lengend Snippet: Time-course of gene expression related to the unfolded protein response within human neurons infected by HCoV-OC43. a

Article Snippet: First, we evaluated the susceptibility of the two well-characterized differentiated human neuronal cell lines NT2-N ( ) and LA-N-5 ( ) to HCoV-OC43 (ATCC strain) infection.

Techniques: Gene Expression, Infection, Microarray

Journal:

Article Title: Maintenance of caspase-3 proenzyme dormancy by an intrinsic "safety catch" regulatory tripeptide

doi: 10.1073/pnas.111085198

Figure Lengend Snippet: Acidification disables the safety catch and promotes procaspase-3 activation. (a) Ac-DEVD-AMC cleavage activity in procaspase-3 expressing bacterial extracts after incubation in buffers at varying pH levels. (b) α-Caspase-3 immunoblot and corresponding Ac-DEVD-AMC cleavage activity in cytosolic extracts from the neuronal precursor cells NT2 treated as described in a.

Article Snippet: Lysates were prepared from the E. coli strain BL21(DE3) pLysS as described above or from the human neuronal precursor cell line NT2 (Stratagene) according to the procedure described ( 6 ).

Techniques: Activation Assay, Activity Assay, Expressing, Incubation, Western Blot